Transcript levels, alternative splicing and proteolytic cleavage of TFIIIA control 5S rRNA accumulation during Arabidopsis thaliana development
نویسندگان
چکیده
منابع مشابه
Regulation of Arabidopsis thaliana 5S rRNA Genes.
The Arabidopsis thaliana genome comprises around 1,000 copies of 5S rRNA genes encoding both major and minor 5S rRNAs. In mature wild-type leaves, the minor 5S rRNA genes are silent. Using different mutants of DNA methyltransferases (met1, cmt3 and met1 cmt3), components of the RNAi pathway (ago4) or post-translational histone modifier (hda6/sil1), we show that the corresponding proteins are ne...
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In eukaryotes, genes transcribed by RNA polymerase III (Pol III) carry their own internal promoters and as such, are transcribed as individual units. Indeed, a very few cases of dicistronic Pol III genes are yet known. In contrast to other hemiascomycetes, 5S rRNA genes of Yarrowia lipolytica are not embedded into the tandemly repeated rDNA units, but appear scattered throughout the genome. We ...
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Alternative splicing is an important process for increasing the diversity arising from a single gene. Presently, most studies aimed at detecting alternatively spliced genes use Expressed Sequence Tags (ESTs). However, the EST studies based on spliced transcripts analyse sequences by alignment rather than sequence patterns. Second, EST libraries can be of uncertain quality. To address these issu...
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Background Alternative splicing is the major post-transcriptional mechanism by which gene expression is regulated and affects a wide range of processes and responses in most eukaryotic organisms. RNA-sequencing (RNA-seq) can generate genome-wide quantification of individual transcript isoforms to identify changes in expression and alternative splicing. RNAseq is an essential modern tool but its...
متن کاملThe role of zinc finger linkers in p43 and TFIIIA binding to 5S rRNA and DNA.
Transcription factor IIIA (TFIIIA) and p43 zinc finger protein form distinct complexes with 5S ribosomal RNA in Xenopus oocytes. Additionally, TFIIIA binds the internal promoter of the 5S RNA gene and supports assembly of a transcription initiation complex. Both proteins have nine tandemly repeated zinc fingers with almost identical linker lengths between corresponding fingers, yet p43 has no d...
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ژورنال
عنوان ژورنال: The Plant Journal
سال: 2012
ISSN: 0960-7412
DOI: 10.1111/j.1365-313x.2012.04948.x